the study of chromosome constitution in secondary tritipyrum genotypes (f2) by genomic in situ hybridization technique (gish)
نویسندگان
چکیده
to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of this f2 progeny was assessed by genomic in situ hybridization (gish). the results indicated that 1) without using chinese spring dna as a blocker differentiation between a, b, d and eb chromosomes was impossible, 2) secondly differentiation between a, b, d and eb informs of single or pairs (rod or ring) chromosomes was improved by adding the preblocking hybridization step, using chinese spring genomic dna as probe, to normal gish protocol. third the eb chromosome counts in f2 plants showed the feasibility of selection of the so called secondary tritipyrum genotypes (f2) with different number of d and eb chromosomes for favorable agronomic traits by genomic in situ hybridization as a powerful cytogenetic assisted marker selection (cams).
منابع مشابه
Production and First Assessment of Iranian Secondary Tritipyrum Genotypes (ISTG) by GISH and AFLP Markers in Iran and Germany
Background: Non-Iranian Primary Tritipyrum (2n=6x=42, AABBEbEb) set seed after Triticale (2n=6x=42, AABBRR) and Tritordeum (2n=6x=42, AABBHcHc) but, due to a few undesirable agronomic traits, it cannot fulfil the commercial expectations of farming. Objectives: To remove these deficiencies, six hexaploid Tritipyrum lines were crossed with four Iranian bread wheat cultivars which led to the...
متن کاملشناسایی ژنوتیپهای تریتی پایرم ثانویه با هیبریداسیونDNA ژنومی در محل (GISH)
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متن کاملشناسایی ژنوتیپهای تریتی پایرم ثانویه با هیبریداسیونDNA ژنومی در محل (GISH)
The genomic in situ hybridization (GISH) has been used to identify euploidy and aneuploidy in segregation generations of various plants. In this study, the GISH with minor modifications including, slide preparation of putative secondary Tritipyrum (F2) root meristemic cells, labeled genomic DNA of Thinopyrum bessarabicum by fluorescein 12-dUTP nucleotide as probe, genomic DNA of Thinopyrum bess...
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عنوان ژورنال:
پژوهش های تولید گیاهیجلد ۱۷، شماره ۲، صفحات ۹۳-۱۰۸
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